Sperm quality parameters of Iberian toothcarp (Aphanius iberus) and Valencia toothcarp (Valencia hispanica): New conservation tools from a gamete perspective

The sensitive state of conservation several endemic fish species such as Iberian toothcarp (Aphanius iberus) and Valencia (Valencia hispanica) has led governments to consider the implementation measures preserve their populations. However, limited knowledge about reproductive biology these makes it necessary investigate different aspects cycle. In this sense, main objectives work were i) advance breeding both species, ii) develop protocols for gametes future management conservation. During spring 2019 a temporal series samplings carried out in places Comunitat Valenciana. Sperm samples collected sperm motion parameters assessed first time species. Kinetic patterns similar showing high motility velocity values during 30 s, rapid decrease from that point. At same time, an in-depth morphometric analysis was using computer-assisted software. Spermatozoa A. iberus V. hispanica showed sizes shapes other external fertilizers belonging Cyprinodontiformes, with small spherical heads, uniflagellated without acrosomes. addition, new cryopreservation protocol designed cryobanking threatened Cryopreserved lower than fresh but reaching acceptable percentages motile cells after thawing around 20 25% (A. hispanica, respectively). This study is its kind successfully achieve gamete two endangered Peninsula, providing useful tools complement programs are being developed